标题:Use of quantitative PCR with the chloroplast gene rps4 to determine moss abundance in the early succession stage of biological soil crusts作者:Deng, Songqiang; Wang, Chunzi; De Philippis, Roberto; Zhou, Xiangjun;Ye, Chaoran; Chen, Lanzhou
来源出版物:BIOLOGY AND FERTILITY OF SOILS 卷:52 期:5 页码:595-599 DOI: 10.1007/s00374-016-1107-7 出版年:JUL 2016
摘要:The quantitative PCR (qPCR) method was firstly used to measure moss abundance with moss chloroplast gene rps4 as compared to that with cyanobacterial 16S rRNA gene and fungal 25-28S rRNA gene in the early succession stage of biological soil crusts (BSCs). Four sites with three BSC types collected from Hobq Desert of China, representing cyanobacterial-, lichen-, and moss-dominated BSCs were investigated. The copies of the moss rps4 gene, cyanobacterial 16S rRNA gene, and fungal 25-28S rRNA gene, chlorophyll a content, and the community composition variated significantly. The moss rps4 gene copies attained a significant positive correlation with chlorophyll a content and showed a cross-validation with relative moss biomass.
入藏号:WOS:000377994900001
文献类型:Article
语种:English
作者关键词:Moss, Quantitative PCR, rps4, Biomass, Community composition
扩展关键词:16S RIBOSOMAL-RNA; MOJAVE DESERT; BACTERIAL; POLYSACCHARIDES; CYANOBACTERIA; COMMUNITIES; PHYLOGENY; DIVERSITY; PRIMERS; BIOMASS
通讯作者地址:Chen, LZ, Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Wuhan 430079, Peoples R China.
电子邮件地址:
地址:
[Deng, Songqiang; Wang, Chunzi; Zhou, Xiangjun; Ye, Chaoran; Chen, Lanzhou] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Wuhan 430079, Peoples R China.
[De Philippis, Roberto] Univ Florence, Dept Agrifood Prod & Environm Sci, Piazzale Cascine 24, I-50144 Florence, Italy.
研究方向:Agriculture
ISSN:0178-2762
eISSN: 1432-0789
影响因子:3.069
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