标题: Copper regulates degradation of typical antibiotics by microalgal-fungal consortium in simulated swine wastewater: insights into metabolic routes and dissolved organic matters

作者: Li, SX (Li, Shuangxi); Zhu, LD (Zhu, Liandong)

来源出版物: WATER RESEARCH 卷: 245 文献号: 120654 DOI: 10.1016/j.watres.2023.120654 出版年: OCT 15 2023

摘要: Microalgae-based biotechnology for antibiotics biodegradation in swine wastewater has been receiving an increasing attention. In this study, microalgae and fungi co-cultivation system, regulated by copper (Cu(II)), was investigated in terms of nutrients and sulfonamides degradation in simulated swine wastewater. Results showed that the removal of ammonium nitrogen (NH4+-N), total nitrogen (TN), total phosphorus (TP) and chemical oxygen demand (COD) by microalgal-fungal consortium increased under 0.1-0.5 mg/L Cu(II) with the highest removal efficiency of 79.19%, 76.18%, 93.93% and 93.46%, respectively. The addition of Cu(II) (0-0.5 mg/L) enhanced the removal of sulfamonomethoxine (SMM), sulfamethoxazole (SMX) and sulfamethazine (SMZ) from 49.05% to 58.76%, from 59.31% to 63.51%, and from 37.51% to 63.9%, respectively, and the main removal mechanism was found to be biodegradation. Biodegradation followed a pseudo-first-order model with variable half-lives (10.12 to 15.51 days for SMM, 9.01 to 10.88 days for SMX, and 8.74 to 12.85 days for SMZ). Through mass spectrometry analysis, metabolites and intermediates of sulfonamides were accordingly identified, sug-gesting that the degradation routes were involved with hydroxylation, deamination, oxidation, de-sulfonation and bond cleavage. Dissolved organic matters released by microalgal-fungal consortium were induced by Cu (II). Fulvic acid-like and protein-like substances were bound to Cu(II), reducing its concentration and thus mitigating the organismal damage to microorganisms. These findings drew an insightful understanding of microalgal-fungal consortium for sulfonamides remediation by Cu(II) regulation in simulated swine wastewater.

作者关键词: Copper; Degradation pathway; Dissolved organic matters; Microalgal-fungal consortium; Sulfonamides

地址: [Li, Shuangxi; Zhu, Liandong] Wuhan Univ, Sch Resources & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.

通讯作者地址: Zhu, LD (通讯作者)，Wuhan Univ, Sch Resources & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.

电子邮件地址: ldzhu@whu.edu.cn

影响因子：12.8